Laboratory of Protein Biosynthesis

Head Professor Boris S. Negrutskii, Dr.Sc. Phone: (380-44) 526-43-97; Fax: (380-44) 526-07-59; E-mail: negrutskii@imbg.org.ua

Науковий напрямок:

The studies of the Laboratory are focused on the factors and molecular mechanisms ensuring the high efficiency and accuracy of higher eukaryotic genome expression at the transcription and translation stages.

Our current research activities are going on in following directions:

• i) Analysis of the mechanisms of dynamic compartmentalization of protein synthesis apparatus in Mammalians. Investigation of the role of translation elongation factor 1A (eEF1A) in protein biosynthesis and other processes in mammalian cells.
  Recent achievements.
  • We have established the concept of vectorial transfer of tRNA during the translation elongation process in mammalian cells.
  • The channeling of deacylated tRNA has been found to occur via the formation of non-canonic complexes of eEF1A with tRNA and aminoacyl-tRNA synthetases.
  • The evidence has been found that eEF1A belongs to the family of partially unstructured proteins.
  • eEF1A and ribosomes are found to possess chaperon-like properties which are important for maintaining active conformation of protein components of translation apparatus.
• ii) Investigation of the molecular processes involved into the transition of undividable cells to proliferation. Studies on the mechanisms regulating the activity of detoxication system in human placenta.
  Recent achievements.
  • We have proposed time-scale of regeneration process based on the proliferative activity and cytokine synthesis by both parenchymatic and non-parenchymatic liver cells.
  • We were the first to show the involvement of interferon alpha into transition of cells of regenerating liver from "rest" (from proliferation point of view) to active proliferation.
  • Efficiency of detoxication in human placenta has been found dependent on the effect that environmental hazards have on mother's organism as well as polymorphism type in placenta detoxication genes.

Selected publications:

1. Lukash T.O., Turkivska H.V., Negrutskii B.S., El’skaya F.V. (2004) Chaperone-like activity of mammalian elongation factor eEF1A: renaturation of aminoacyl-tRNA synthetases. Int J Biochem Cell Biol. 36(7):1341-1347.
2. Budkevich, T.V., Timchenko, A. A., Tiktopulo, E.I., Negrutskii, B.S., Shalak, V.F., Petrushenko, Z.M., Aksenov, V.L., Willumeit, R., Kohlbrecher, J., Serdyuk, I.N., El’skaya, A.V. (2002) Extended conformation of mammalian translation elongation factor 1A in solution Biochemistry 41, 15342-15349.
3. Petrushenko Z.M., Budkevich T.V., Shalak V.F., Negrutskii B.S., El’skaya A.V. (2002) Novel complexes of mammalian translation elongation factor eEF1A*GDP with uncharged tRNA and aminoacyl-tRNA synthetase. Implications for tRNA channeling. Eur. J. Biochem. 269, 4811-4818.
4. Negrutskii B.S., Shalak V.F., Kerjan P., El’skaya A.V., Mirande M. (1999) Functional interaction of mammalian valyl-tRNA synthetase with elongation factor EF-1A in the complex with EF-1H. J.Biol. Chem. 274, 4545-4550.
5. Negrutskii B.S., El’skaya A.V. (1998) Eukaryotic translation elongation factor 1 alpha: structure, expression, functions, and possible role in aminoacyl-tRNA channeling. Progr.Nucl.Acids Res.Mol.Biol. 60, 47 – 78.
6. Petrushenko Z.M., Negrutskii B.S., Ladokhin A.S., Budkevich T.V., Shalak V.F., El’skaya A.V. (1997) Evidence for the formation of an unusual ternary complex of rabbit liver EF-1а with GDP and deacylated tRNA. FEBS Lett. 407, 13-17.
7. Negrutskii B. S., Budkevich T. V., Shalak V. F., Turkovskaya G. V., El’skaya A. V. (1996) Rabbit translation elongation factor 1 alpha stimulates the activity of homologous aminoacyl-tRNA synthetase. FEBS Lett. 382, 18-20.
8. Obolenskaya M.Yu., Schulze-Specking A., Plaumann B., Frenzer K., Freudenberg N. and K. Decker. (1994) Nitric oxide production by cells isolated from regenerating rat liver. Biochem.Biophys. Res. Comm. - V. 204, № 3. - Р. 1305 –1311.
9. Obolenskaya M.Yu. (1997) Cytokines and liver regeneration. - EOS - J. Immunology and Immunopharmacology. - V.17, № 2. - Р. 51 -58.
10. Obolenskaya M.Yu., Samoylenko A.A. (2000) Does nitric oxide regulate the transduction of TNF signal?- EOS - J. Immunology and Immunopharmacology. - V.20, № 1. - Р. 13-20.
11. Budkevich TV, El'skaya AV, Nierhaus KH. Features of 80S mammalian ribosome and its subunits. Nucleic Acids Res. 2008;36(14):4736-44.
12. Panasyuk G., Nemazanyy I., Filonenko V., Negrutskii B., El'skaya A. A2 isoform of mammalian translation factor eEF1A displays increased tyrosine phosphorylation and ability to interact with different signalling molecules Int J Biochem Cell Biol. 2008;40(1):63-71.
13. Kanibolotsky DS, Novosyl'na OV, Abbott CM, Negrutskii BS, El'skaya AV. Multiple molecular dynamics simulation of the isoforms of human translation elongation factor 1A reveals reversible fluctuations between "open" and "closed" conformations and suggests specific for eEF1A1 affinity for Ca2+-calmodulin. BMC Struct Biol. 2008;8:4.