Department of Translation Mechanisms of Genetic Information

Head

Anna V. El’skaya

Professor, Dr. Sci. (Mol. Biol.),
Member of National Academy of Sciences of Ukraine Phone: (380-44) 526- 07-49;
Fax: (380-44) 526-07-59;
E-mail: elskaya@imbg.org.ua

Laboratory of Biomolecular Electronics

Head: Olexey P. Soldatkin,
Professor, Dr. Sci. (Biotech.), Corresponding Member of NASU

Laboratory of Protein Biosynthesis

Head: Boris S. Negrutskii,
Professor, Dr. Sci. (Mol. Biol.)

Education and Degrees:

1963 Graduate Student, Maxim Gorky Donetsk National Medical University, Donetsk, Ukraine, M.D.

1968 Ph.D. (molecular biology), O. V. Palladin Institute of Biochemistry (IBC), NASU, Kyiv, Ukraine

1976 Dr.Sci. (molecular biology), IBC NASU, Kyiv, Ukraine

1986 Full Professor, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

1988 Corresponding Member of NASU (molecular biology)

1992 Full Member of NASU (molecular biology)

Professional Employment:

1963–1965 Senior Laboratory Assistant, Maxim Gorky Donetsk National Medical University, Donetsk, Ukraine

1965–1968 Postgraduate Student, O. V. Palladin Institute of Biochemistry (IBC) NASU, Kyiv, Ukraine

1968–1973 Junior Research Scientist, IBC NASU, Kyiv, Ukraine

1973–1978 Senior Research Scientist, Institute of Molecular Biology and Genetics (IMBG), NASU, Kyiv, Ukraine

1978–1996 Head of Department, IMBG NASU, Kyiv, Ukraine

1996–2003 Deputy Director, IMBG NASU, Kyiv, Ukraine

since 2003 Director, IMBG NASU, Kyiv, Ukraine

Membership:

Vice-President of Ukrainian Biochemical Society

Head of the Expert committee on biology of Ministry of Education and Science of Ukraine

Head of the Scientific Council of IMBG NASU

Head of NASU Scientific and Technical Program "Sensor systems for medico-ecological and industrial-technological requirement: metrological support and experimental operation"

Head of the Scientific Expert Group for the “Fundamentals of Molecular and Cellular Biotechnology” Program of NASU

Editor-in-Chief of Journal “Biopolymers and Cell” (Ukraine)

Editorial Board member of Journals: “Ukrainica Bioorganica Acta” (Ukraine), “Bulletin of Vavylov

Society of Geneticists and Breeders of Ukraine” (Ukraine)

Editorial Council member of Journals: “Ukrainian Biochemical Journal” (Ukraine), “Biotechnologia Acta” (Ukraine)

Honours, Prizes, Awards:

1976 O. V. Palladin Award of National Academy of Sciences of Ukraine

1982 Diploma of Presidium of Verkhovna Rada of Ukrainian SSR

1986 State Prize of Ukraine in Science and Technology

1989 Diploma of State Committee for Inventions and Discoveries of USSR

1998 Honoured Worker of Science and Technology of Ukraine

2004 Diploma of Verhovna Rada of Ukraine

2005 Gold Medal of the Ukrainian Federation of Scientists

2008 The Order of Princess Olga, III degree

2009 “European Quality Award” and Europe Business Assembly Diploma

2012 The Order of St. Andrew, III degree

2013 Gratitude of the Prime Minister of Ukraine

2015 The Order of Princess Olga, II degree

2016 Vernadsky Gold Medal of the National Academy of Sciences of Ukraine for outstanding achievements in the field of molecular biology and bioelectronics

2017 "Woman of Ukraine 2016" Award, nomination "Science and Education"

Research Area:

The mechanisms of protein synthesis and its regulation in higher eukaryotes

The development of novel analytical systems, bio- and chemosensors, on the basis of different transducers and biological material or synthetic biomimics

Ñurrent Research Activities and Recent Achievements:

  • The concept of vectorial transfer of tRNA during translation elongation process in mammalian cells has been established. Molecular mechanisms of tRNA channeling have been uncovered.
  • Spatial structures of A1 and proto-oncogenic A2 isoforms of mammalian eEF1 have been shown to be different despite 97 % homology of their amino acid sequences. Differential interaction of the isoforms with protein partners has been demonstrated which might be important for oncogenecity of A2.
  • X-ray structure of the proto-oncogenic A2 isoform has been obtained, in collaboration with Department of Protein Synthesis Enzymology, and some peculiariries in the mechanism of GTP/GDP exchange have been reveiled.
  • Mechanism of overexpression of A2 in tumors has been shown to be related to special microRNAs whose role has been predicted theoretically and proved experimentally.
  • The components of macromolecular eEF1 complex have been shown to function independently from the complex in human cancer cells and tissues where the subunits may apparently fulfill a non-translational role.
  • Development and creation of micro/nano electrochemical bio- and chemosensors for the in vitro and in vivo determination of major metabolites, some neurotransmitters, alcohols, aldehydes and toxic substances.
  • Use of nanomaterials, nanotubes and zeolites to improve analytical characteristics of bio- and chemosensor devices.
  • Use of new genetically engineered proteins and synthetic recognition molecules to create sensors with given analytical characteristics.
  • Creation of synthetic analogues of biological receptors by molecular imprinting (molecularly imprinted polymers – MIP) and their introduction in sensor technology, solid phase extraction and chromatography.
  • Development of electrochemical and optical affine (immune- and DNA-) sensors.
  • Study on fundamentals of interaction of immobilized enzymes with substrates and inhibitors using electrochemical sensors.

Selected publications:

  1. Marchenko S, Kucherenko I, Hereshko A, Panasiuk I, Soldatkin OO, El'skaya A, Soldatkin AP. Application of potentiometric biosensor based on recombinant urease for urea determination in blood serum and hemodialyzate. Sensors and Actuators B: Chemical, 2015, V. 207, P. 981-986.
  2. Vlasenko D, Novosylna O, Negrutskii B,...El'skaya A. Truncation of the A,A(∗),A' helices segment impairs the actin bundling activity of mammalian eEF1A1. FEBS Lett. 2015; 589(11):1187-93.
  3. Veremieva M, Kapustian L, Khoruzhenko A, Zakharychev V, Negrutskii B, El'skaya A. Independent overexpression of the subunits of translation elongation factor complex eEF1H in human lung cancer. BMC Cancer. 2014 Dec 3;14:913.
  4. Trosiuk TV, Liudkovska VV, Shalak VF, Negrutskii1 BS, El’skaya AV. Structural dissection of human translation elongation factor 1Bγ (eEF1Bγ): expression of full-length protein and its truncated forms. Biopolymers and Cell. 2014;30(2):96-106.
  5. Thibaut Crepin, Shalak VF, Yaremchuk AD, ...El'skaya AV. Mammalian translation elongation factor eEF1A2: X-ray structure and new features of GDP/GTP exchange mechanism in higher eukaryotes. Nucleic Acids Research. 2014;42(20):12939-48.
  6. Sergeyeva TA, Chelyadina DS, Gorbach LA, ...El'skaya AV. Colorimetric biomimetic sensor systems based on molecularly imprinted polymer membranes for highly-selective detection of phenol in environmental samples. Biopolymers and Cell. 2014;30(3):209-216.
  7. Soldatkin OO, Kasap OB, Akata B,...El'skaya AV. Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation. Biopolymers and Cell. 2014;30(4):291-298.
  8. Vislovukh A, Kratassiouk G, Porto E, Gralievska N, Beldiman C, Pinna G, El’skaya A, Harel-Bellan A, Negrutskii B, Groisman I. Proto-oncogenic isoform A2 of eukaryotic translation elongation factor eEF1 is a target of miR-663 and miR-744 // Br J Cancer -2013 Jun 11; N108. - V.11 P. 2304–11.
  9. T.A.Sergeyeva, L.A.Gorbach, E.V.Piletska...A.V.El’skaya. Colorimetric test-systems for creatinine detection based on composite molecularly imprinted polymer membranes // Analytica Chimica Acta. -2013. - V. 770. -P.161–168
  10. A.P. Soldatkin, S. Dzyadevych, T. Sergeeva, Y. Korpan... El’skaya A.V. Biosensors. A quarter of a century of R&D experience // Biopolymers and cell. -2013. –V. 29. N 3. –P. 188-206
  11. SoldatkinÎ.Î., KucherenkoI.S., PyeshkovaV.M., KuklaA.L., Jaffrezic-RenaultN., El’skaya A.V.,Dzyadevych S.V., SoldatkinA.P. Conductometric biosensor based on three- enzyme system for selective determination of heavy metal ions // Bioelectrochemistry. – 2012. – V.30. – P. 25-30.
  12. Slinchenko O., Gorbach L., Matyushov V., Brovko O., Piletsky S., Sergeeva L., El’ska G., Sergeyeva T. Catalytic molecularly imprinted polymer membranes: Development of the biomimetic sensor for phenols detection // Anal. Chim. Acta. – 2010. – V.659,N1-2. – P. 274-279.
  13. Sergeyeva T., Gorbach L., Slinchenko O., Goncharova L., Piletska O., Brovko O., Sergeeva L., El’ska G. Towards development of colorimetric test- systems for phenols detection based on computationally-designed molecularly imprinted polymer membranes // Material Science and Engineering C. – 2010. – V.30. – P. 431–436.
  14. Veremieva M., Khoruzhenko A., Zaicev S., Negrutskii B., El’skaya A. Unbalanced expression of the translation complex eEF1 subunits in human cardioesophageal carcinoma // European Journal of Clinical Investigation. – 21 October 2010. – Published on-line.
  15. Tokovenko B., Golda R., Protas O., Obolenskaya M., El'skaya A. COTRASIF: conservation-aided transcription-factor-binding site finder // Nucleic Acids Res. – 2009. – V.37, N7. – e 49.
  16. Budkevich TV, El'skaya AV, Nierhaus KH. Features of 80S mammalian ribosome and its subunits // Nucleic Acids Res. - 2008. - V.36,N14. - P. 4736-44.
  17. Panasyuk G., Nemazanyy I., Filonenko V., Negrutskii B., El'skaya A. A2 isoform of mammalian translation factor eEF1A displays increased tyrosine phosphorylation and ability to interact with different signalling molecules // Int J Biochem Cell Biol. - 2008. - V.40,N1. - P. 63-71.
  18. Kanibolotsky DS, Novosyl'na OV, Abbott CM, Negrutskii BS, El'skaya AV. Multiple molecular dynamics simulation of the isoforms of human translation elongation factor 1A reveals reversible fluctuations between "open" and "closed" conformations and suggests specific for eEF1A1 affinity for Ca2+-calmodulin // BMC Struct Biol. - 2008. - V.8,N4.
  19. S.V.Dzyadevych, V.N.Arkhypova, A.P.Soldatkin, A.V.El’skaya, C.Martelet, N.Jaffrezic-Renault. Conductometric enzyme biosensors // Handbook of Biosensors and Biochips / Eds R.S.Marks, C.R.Lowe, D.C.Culen, H.H.Weetall, I.Karube.- Wiley-Interscience. - 2007. - P. 379-394.
  20. S.V.Dzyadevych, A.P.Soldatkin, A.V.El’skaya, C.Martelet, N.Jaffrezic-Renault. Enzyme biosensors based on ion-selective field-effect transistors // Analytica Chimica Acta.- 2006. - V.568.- P. 248-258.
  21. A.P.Soldatkin, S.V.Dzyadevych, A.V.El’skaya, C.Martelet, N.Jaffrezic-Renault. Pathways for improving potentiometric and conductometric enzymatic biosensors // In: Encyclopedia of Sensors (Eds. C.A.Grimes, E.C.Dickey, M.V.Pishko).- California, USA: American Scientific Publishers. - 2006. - V.7.- P. 331-348.
  22. S.V.Dzyadevych, A.P.Soldatkin, V.N.Arkhypova, C.Martelet, N.Jaffrezic-Renault, A.V.El’skaya. Electrochemical enzyme biosensors // Kyiv: IMBG. - 2006. - 72 p.
  23. A.P.Soldatkin, S.V.Dzyadevych, A.V.El'skaya, C.Martelet, N.Jaffrezic-Renault. Pathways for improving potentiometric and conductometric enzymatic biosensors // Encyclopedia of Sensors, American Scientific Publishers, submitted, 2005.
  24. A.P.Soldatkin, V.N.Arkhypova, S.V.Dzyadevych, A.V.El'skaya, J.-M.Gravoueille, N.Jaffrezic-Renault, C.Martelet. Analysis of the potato glycoalkaloids by using of enzyme biosensor based on pH-ISFETs // Talanta. - 2005. - V.66 - P.28-33.
  25. S.V.Dzyadevych, A.P.Soldatkin, V.N.Arkhypova, A.V.El'skaya, J.-M. Chovelon, C.Georgiou, C.Martelet, N.Jaffrezic-Renault. Early-warning electrochemical biosensor system for the environmental monitoring based on enzyme inhibition effect // Sensors and Actuators B. - 2004.
  26. V.N.Arkhypova, S.V.Dzyadevych, A.P.Soldatkin, Y.I.Korpan, A.V.El'skaya, J.-M.Gravoueille, C.Martelet, N.Jaffrezic-Renault. Application of enzyme field effect transistors for fast detection of total glycoalkaloids content in potatoes // Sensors and Actuators B. - 2004.- V.103.- P. 416-422.
  27. O.A.Biloivan, S.V.Dzyadevych, O.A.Boubryak, A.P.Soldatkin, A.V.El'skaya. Development of enzyme biosensor based on ISFETs for quantitative analysis of serine proteinases // Electroanalysis. - 2004. - V.16. - P. 1883-1889.
  28. Lukash, T.O. Turkivska H.V., Negrutskii B.S., El'skaya A.V. Chaperone-like activity of mammalian elongation factor eEF1A: Renaturation of aminoacyl-tRNA synthetases // International Journal of Biochemistry & Cell Biology. - 2004. - V.36. - P. 1341-1347
  29. Korpan Y.I., Nazarenko E.A., Skryshevskaya I.V., Martelet C., Jaffrezic-Renault N., El'skaya A.V., Potato Glycoalkaloids: True Safety or False Sense of Security? // Trends in Biotechnology. - 2004. - V.22,N3. - P. 147-151.
  30. S.V.Dzyadevych, V.N.Arkhypova, A.P.Soldatkin, A.V.El'skaya, C.Martelet, N.Jaffrezic-Renault. Enzyme biosensor for tomatine detection in tomatoes // Analytical Letters.- 2004. - V.37,N8. - P. 1611-1624.
  31. S.V.Dzyadevych, V.N.Arkhypova, C.Martelet, N.Jaffrezic-Renault, J.-M.Chovelon, A.V.El'skaya, A.P.Soldatkin. Potentiometric biosensors based on ISFETs and immobilized cholinesterases // Electroanalysis. - 2004. - V.16. - P. 1873-1882.
  32. T.V. Budkevich, A.A. Timchenko, E.I. Tiktopulo, B.S. Negrutskii, V.F. Shalak, Z.M. Petrushenko, V.L. Aksenov, R. Willumeit, J. Kohlbrecher, I.N.Serdyuk, A.V.El'skaya. Extended conformation of mammalian translation elongation factor 1A in solution // Biochemistry. - 2003. - V.24,N51. - P.15342-15349.
  33. T.A.Sergeyeva, S.A.Piletsky, E.V.Piletska, O.O.Brovko, L.V.Karabanova, L.M.Sergeeva, A.V.El'skaya, A.P.F.Turner. In situ formation of porous molecularly imprinted polymer membranes // Macromolecules. - 2003.- V.36,N19. - P. 7352-7357.
  34. V.N.Arkhypova, S.V.Dzyadevych, A.P.Soldatkin, A.V.El'skaya, C.Martelet, N.Jaffrezic-Renault. Development and optimisation of biosensors based on pH-sensitive field effect transistor and cholinesterase for sensitive detection of solanaceous glycoalkaloids // Biosensors & Bioelectronics. - 2003. - V.18. - P. 1047-1053.
  35. S.V.Dzyadevych, A.P.Soldatkin, Y.I.Korpan, V.N.Arkhypova, A.V.El'skaya, J.-M.Chovelon, C.Martelet, N.Jaffrezic-Renault. Biosensors based on enzyme field effect transistors for determination of some substrates and inhibitors // Analytical and Bioanalytical Chemistry. - 2003. - V.377. - P. 496-506.
  36. Petrushenko Z.M., Budkevich T.V., Shalak V.F., Negrutskii B.S., El'skaya A.V. Novel complexes of mammalian translation elongation factor eEF1A*GDP with uncharged tRNA and aminoacyl-tRNA synthetase. Implications for tRNA channeling // Eur. J. Biochem. - 2002. - V.269. - P. 4811-4818.
  37. Budkevich, T.V., Timchenko, A. A., Tiktopulo, E.I., Negrutskii, B.S., Shalak, V.F., Petrushenko, Z.M., Aksenov, V.L., Willumeit, R., Kohlbrecher, J., Serdyuk, I.N., El'skaya A.V. Extended conformation of mammalian translation elongation factor 1A in solution // Biochemistry. - 2002. - V.41. - P. 15342-15349.