Department of Molecular Genetics

Head

Gennadiy D. Telegeev

Ph.D. (Mol. Genetics), Senior Staff Scientist
Phone: (380-44) 526-07-29;
Fax: (380-44) 526-07-59;
E-mail: g.d.telegeev@imbg.org.ua

Education and Degrees:

19681974 Graduate Student, Bogomolets National Medical University, Kyiv, Ukraine

19781982 Ph.D. Student, Department of Gene Engineering, Institute of Molecular Biology and Genetics (IMBG), NASU, Kyiv, Ukraine

1990 Ph.D. (molecular biology)

2013 Dr.Sci. (molecular biology)

Professional Employment:

19771984 Engineer, Department of Gene Engineering, IMBG NASU, Kyiv, Ukraine

19841992 Junior Research Scientist, Department of Gene Engineering, IMBG NASU, Kyiv, Ukraine

19921998 Research Scientist, Department of Gene Engineering, IMBG NASU, Kyiv, Ukraine

19982008 Senior Research Scientist, Department of Molecular Genetics, IMBG NASU, Kyiv, Ukraine

since 2008 Head of the Department of Molecular Genetics, IMBG NASU, Kyiv, Ukraine

Research Area:

Investigation of factors and molecular mechanisms of malignant transformation in human

Development of technology of targeted drug delivery to eukaryotic cells

urrent Research Activities and Recent Achievements:

  • Study on molecular pathogenesis of chronic myelogenous leukemia (CML) and role of Bcr domains in this pathology.
  • Elaboration of the test-system for Ph-positive leukemias diagnostics and detection of Abl mutations in patients with resistance to imatinib.
Fig 1. Proteomic analysis of proteins that interact with the PH domain of Bcr-Abl(A) SDS-PAGE analysis of purification of PH domain of Bcr- Abl with histidine tag (amino acid residues 674-869). Visualization was carried out using the dye Coomassie brilliant blue B) Twodimensional gel electrophoresis of total cellular proteins from the K562 cell culture. PH gradient separation in the first dimension and SDS-PAGE direction are shown on the top and on the right side of the gel, respectively. Images of two-dimensional gels of proteins that were co-precipitated with PH domain containing histidine tag (C)). Two-dimensional gel electrophoresis of total cellular proteins containing His tag or with His tag alone (D). Silver staining (left side panel) and 35S-labeled proteins (right side panel)
  • Formulating novel role of PH domain as protein-protein and protein-lipid anchor, study on proteins that interact with it as well as changes in methylation status of the promotor regions of certain genes in cancer.
  • Development of nanovectors on the basis of branched dextran-polyacrilamide and dispersed silicium for targeted drug delivery to cells with phagocyte activity as a step for therapy of monocytic leukemias and histiocytic neoplasms.
  • Studies on CXCR4 expression in cancer progenitor cells and development of nanotechnology-based therapy and imaging for prostate cancer using CXCR4-targeted metal nanoparticles.
Fig 2. Interaction of p210 Bcr-Abl with the Golgi complex. (A) Cos-1 cells transfected with p190Bcr-Abl and p210Bcr-Abl. Immunofluorescent staining: Bcr-Abl-anti-Abl antibody AlexaFluor488-labeled secondary antibodies, Detection of Golgi apparatus was performed by using monoclonal antibodies against GM130, secondary antibodies TRITC-conjugated murine antibodies. Dash 20 mkm. The arrow shows the Golgi apparatus B) Assessment of inhibition of PTEN protein by specific sh RNA (specific anti-PTEN antibodies were used for the detection) C) Evaluating the effectiveness of inhibition of sh RNA specific to PI4K using real-time PCR. D) Effect of sh RNA specific to PI4K on localization of p210 Bcr-Abl and p190Bcr-Abl in HEK293 cells. D) Pearson correlation, overlapping p210 Bcr-Abl and p190Bcr- Abl with GM 130

National Grants:

Projects of National Academy of Sciences of Ukraine:

  • 2013 Innovative Project: The development and introduction into medical practice of highly specific molecular genetic diagnostic markers for malignant tumors of the genitourinary system
  • 2012 Innovative Project: The development and introduction into clinical practice the complex immunocytochemical and molecular genetic technologies for diagnosis of acute leukemia
  • 20102014 N 42/10 Project: The study of molecular genetic changes in myeloproliferative diseases and their use in clinical diagnostics

Projects of State Fund for Fundamental Researches:

  • 20102014 N 5.17.2.35 Project: Development of nanotechnology from silica and branched dextran-polyacrylamide polymers to deliver drugs into cells with phagocytic activity as a step towards targeted therapies of monocytic leukemias and histiocytic tumors

Collaboration:

with Ukrainian organizations:

  • National Cancer Institute, Ministry of Health of Ukraine (Kyiv)
  • Kyiv Regional Oncological Dispensary (Kyiv)
  • Taras Shevchenko National University of Kyiv (Kyiv)
  • O. O. Chuiko Institute of Surface Chemistry, NASU (Kyiv)
  • R .E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU (Kyiv)
  • Kyiv Institute for Nuclear Research, NASU (Kyiv)

with foreign organizations:

  • Institute of Biochemistry and Biophysics, PAS (Warsaw, Poland)
  • OncoRay National Center for Radiation Research in Oncology (Dresden, Germany)

Selected publications:

  1. Gridina N.Ya., Schvachko L.P., Draguntsova N.G. Tumor-Associated Inflammation Mechanisms Correction By Verapamil At Brain Gliomas Progression. European Journal of Pharmaceutical and Medical Research (EJPMR). 2016, 3(8): 73-78.
  2. Zimina O.V., Parii M.F., Alkhimova O.G. Loss of heterozygosity at individual loci in Arabidopsis thaliana regenerants cultured with para-fluoropheny lalanine. Cytology and Genetics. 2016, 50 (4):11-15.
  3. Monika Cojoc, Eduard A. Stakhovsky, Gennady D. Telegeev, Anna Dubrovska. Targeting of the epigenetic reprogramming for prostate cancer cell radiosensitization. Cancer research. 2016, 76(9):2637-2651.
  4. S. V. Antonenko, D.S. Gurianov, nd G. D. Telegeev. Colocalization of USP1 and PH Domain of Bcr-Abl Oncoprotein in Terms of Chronic Myeloid Leukemia Cell. Cytology and Genetics. 2016, 5:352-356.
  5. Monika C, Telegeev GT, Dubrovska A. AldegideDehydrogenase Is Regulated by -Catenin/TCF and Promotes Radioresistance in Prostate Cancer Progenitor Cells. Cancer Research. 2015;75(7):1-13.
  6. Polishchuk LA, Telegeev GD. Role of the hybrid Bcr/Abl kinase in the pathogenesis of chronic myeloid leukemia lacking C-Abl and CXCR4 proteins. Exp Oncol. 2014;36(3):138-43.
  7. Cherepenko E, Telegeev G. In search of molecular approaches to improving cancer therapy efficacy. Exp Oncol. 2014;36(1):52-55.
  8. Telegeev GD, Dybkov MV, Dubrovska AN, et al.,Development of molecular oncohematology in Ukraine Biopolym. Cell. 2013; 29(4):277-282 doi:10.7124/bc.000822
  9. Cojoc M, Peitzsch C, Trautmann F, Polishchuk L, Telegeev GD, Dubrovska A. Emerging targets in cancer management: role of the CXCL12/CXCR4 axis. Onco Targets Ther. 2013; 6:134761.
  10. Dubrovska A, Elliott J, Salamone RJ, Telegeev GD, et al. CXCR4 expression in prostate cancer progenitor cells. PLoS One. 2012;7(2):e31226. doi:10.1371/journal.pone.0031226
  11. Miroshnichenko D, Dubrovska A, Maliuta S, Telegeev G, Aspenstrom P. Novel role of pleckstrin homology domain of the BCRABL protein: Analysis of proteinprotein and proteinlipid interactions. Exp Cell Res. 2010;316(4):53042. doi:10.1016/j.yexcr.2009.11.014
  12. Telegeev GD, Dubrovska AN, Nadgorna VA, et al. Immunocytochemical study of Bcr and BcrAbl localization in K562 cells. Exp Oncol. 2010;32(2):813.
  13. Polishchuk LA, Telegeeva PG, Stakhovsky AE, Telegeev GD, Stakhovsky EA. New specific molecular diagnostic markers for Urooncology. Laboratory Diagnostics. 2010;54(4):4651.
  14. Dybkov MV, Gartovska IR, Maliuta SS, Telegeev GD. Detection of V617F mutation of gene jak2 at patients with chronic myeloproliferative neoplasms. Biopolym. Cell. 2010; 26(3):147. doi: 10.7124/bc.000159
  15. Shvachko LP. DNA hypomethylation as Achilles' heel of tumorigenesis: a working hypothesis. Cell Biol Int. 2009; 33(8):90410. doi: 10.1016/j.cellbi.2009.02.018
  16. Rachkov A, Holodova Yu, Ushenin Yu, Miroshnichenko D, Telegeev G, Soldatkin A. Development of bioselective element of Spr spectrometer for monitoring of oligonucleotide interactions and comparison with thermodynamic calculations. Sensor Letters. 2009;7(15):95761. doi: 10.1166/sl.2009.1180
  17. Shvachko LP. Alterations of constitutive pericentromeric heterochromatin in lymphocytes of cancer patients and lymphocytes exposed to 5azacytidine is associated with DNA hypomethylation. Exp Oncol. 2008;30(3):2304.
  18. Telegeev GD, Dubrovska AN, Dybkov MV, Maliuta SS. Influence of BCR/ABL fusion proteins on the course of Ph leukemias. Acta Biochim Pol. 2004;51(3):8459.