Center for Collective Use of Scientific Instruments of IMBG NAS of Ukraine

The Center for Collective Use of Scientific Instruments of IMBG NAS of Ukraine provides access to modern analytical, molecular biology, chromatographic, cytometry, sequencing, and imaging equipment for research in molecular biology, genetics, biochemistry, biotechnology, cell biology, genomics, proteomics, bioinformatics, and biomedical sciences.

The Center supports internal and collaborative research projects, interinstitutional cooperation, scientific and technological developments, educational programs, and may consider applications from external users in accordance with Ukrainian legislation, technical capabilities of the equipment, and approved access procedures.

The Center’s equipment covers key stages of the research workflow: sample preparation and fractionation, HPLC and HPLC-MS analysis, NGS and Sanger sequencing, flow cytometry, gel documentation, chemiluminescent, fluorescent, and phosphor-screen molecular imaging.

Main research applications

  • molecular biology, genetics, and genomics;
  • biochemistry of proteins, nucleic acids, and biomolecular complexes;
  • chromatographic and LC-MS analysis;
  • DNA/RNA sequencing and confirmation of genetic variants;
  • flow cytometry and analysis of cell populations;
  • documentation, quantitative imaging, and analysis of gels, membranes, blots, and phosphor screens;
  • bioinformatics processing, interpretation, and storage of research data.

Possible Areas of Work and Research Collaboration Involving the Research Infrastructure of IMBG NAS of Ukraine

The Center may consider applications not only for access to equipment included in the Center, but also — with the involvement of relevant specialists and structural units of the Institute — for work or research collaboration involving the broader research infrastructure of IMBG NAS of Ukraine in the following areas:

  • next-generation sequencing, genomic, transcriptomic, epigenomic, and metagenomic studies;
  • gene expression analysis, RT-qPCR, digital PCR, development and validation of PCR-based systems;
  • analysis of DNA methylation and other epigenetic modifications;
  • synthesis of DNA/RNA oligonucleotides;
  • expression of recombinant proteins and development of antibodies;
  • rational design of biologically active compounds, chromatography–mass spectrometry analysis, and development of biosensors;
  • creation of databases, bioinformatics support, and use of a high-performance computing cluster;
  • expert support, advanced training, and specialist education.

This list is indicative and not exhaustive. It describes possible areas of work and research collaboration involving the research infrastructure and expertise of IMBG NAS of Ukraine as a whole and is not limited to the current list of instruments included in the Center. The possibility of performing a specific type of work, providing a service, or implementing another form of collaboration is determined after review of the application, taking into account equipment availability, availability of relevant specialists, technical feasibility, requirements for samples and consumables, biosafety, ethics, and organizational conditions.

Cooperation may take the form of work performed by Institute specialists, joint planning and implementation of research projects, expert consultation, analysis of obtained data, or user training. The specific format of cooperation is determined individually, depending on the research task, availability of equipment and personnel, type of samples, requirements for consumables, biosafety, ethics, and technical feasibility.

Access and preparation

To request access to equipment or the performance of work involving the Center, the user should submit an application using the established form. The application should include information about the applicant, institution or organization, user category, basis for using the equipment, project or research topic, source of funding if available, requested equipment, purpose and approximate scope of work, type of samples, need for consumables, need for operator support, and any other technical or organizational requirements.

Submission of an application does not constitute automatic approval of access to equipment or automatic acceptance of the requested work. The application serves as the basis for considering the possibility of access to equipment, reservation of working time, performance of a service, or organization of joint work.

During review of the application, the following are taken into account:

  • the technical capabilities of the specific equipment;
  • compliance of samples with method requirements and safety rules;
  • availability of equipment during the requested period;
  • availability of a responsible person or operator;
  • need for user instruction or knowledge assessment;
  • possibility of independent work by the user on the instrument;
  • need for consumables or reimbursement of their cost;
  • need for operator support, sample preparation, or data processing;
  • funding conditions, agreement terms, or cost reimbursement arrangements;
  • compliance of the planned work with Ukrainian legislation, internal regulations of the Institute, and the Regulations on Access.

The review period for an application does not exceed 30 calendar days from the date of receipt, unless otherwise provided by law, agreement, or the specific nature of the requested work.

Reservation of working time is possible only after review of the application and approval of access. A specific time is reserved through the booking system or by another method designated by the Center. For instruments requiring operator support, prior preparation, or separate approval, the reservation is confirmed by the responsible person or instrument operator. Independent work is allowed only for users who have completed the required instruction and, where necessary, have confirmed their knowledge of the rules for working with the specific instrument.

Access conditions for each instrument are agreed individually, taking into account sample type, sample readiness, required consumables, technical status of the equipment, schedule, safety requirements, need for operator support, and result delivery format.

Documents and contacts

  • Regulations on the Center for Collective Use of Scientific Instruments of IMBG NAS of Ukraine: LINK_TO_FACILITY_REGULATIONS
  • Regulations on Access to the Equipment of the Center for Collective Use of Scientific Instruments: LINK_TO_REGULATIONS
  • Application Form for Access to the Equipment of the Center for Collective Use of Scientific Instruments: LINK_TO_APPLICATION_FORM
  • Laboratory “Center for Collective Use of Scientific Instruments of IMBG NAS of Ukraine” .

The completed application form should be sent by email to: dmitriy.gurianov@edu.imbg.org.ua.

Contact person: PhD, Head of the Laboratory “Center for Collective Use of Scientific Instruments of IMBG NAS of Ukraine” Dmytro Gurianov. Recommended email subject line: Application for Access to the Equipment of the Center for Collective Use of Scientific Instruments of IMBG NAS of Ukraine.

Main Equipment of the Center

Illumina NextSeq 500 Sequencing System for Genomics and Transcriptomics

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Illumina NextSeq 500 Sequencing System for Genomics and Transcriptomics

Illumina NextSeq 500 is a benchtop next-generation sequencing system for high-throughput analysis of prepared DNA and RNA libraries. The platform is used for genomic, transcriptomic, targeted, population-genetic, and molecular-genetic studies followed by bioinformatics analysis.

Capabilities and typical tasks

  • single-end and paired-end sequencing of DNA and RNA libraries;
  • analysis of targeted panels, exomes, transcriptomes, and small or medium-sized genomes;
  • sample multiplexing using indexes;
  • generation of large sequencing datasets for bioinformatics analysis;
  • support for a broad range of NGS projects in molecular biology and genetics.

Key technical specifications

  • compatible with NextSeq 500/550 v2.5 kits;
  • maximum output: up to 120 Gb with the 300-cycle High Output Kit;
  • maximum reads/clusters: up to 400 million for High Output Kits and up to 130 million for Mid Output Kits;
  • support for DNA and RNA libraries;
  • read length up to 2 × 150 bp depending on reagent kit and run configuration.

Access and preparation

Before a run, the library type, concentration, quality, indexes, desired read length, required reagent kit, sequencing depth, primary data format, and need for bioinformatics analysis are agreed. The feasibility of an NGS project depends on library readiness, reagent availability, technical status of the instrument, run schedule, and requirements for data analysis.

Applied Biosystems 3130 Genetic Analyzer for Sanger Sequencing and Fragment Analysis

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Applied Biosystems 3130 Genetic Analyzer for Sanger Sequencing and Fragment Analysis

Applied Biosystems 3130 Genetic Analyzer is a 4-capillary capillary electrophoresis system for Sanger DNA sequencing and fragment analysis. The instrument is used for verification of genetic variants, PCR product analysis, cloning control, and confirmation of selected NGS results.

Capabilities and typical tasks

  • Sanger DNA sequencing;
  • fragment analysis of fluorescently labeled DNA products;
  • verification of selected variants after NGS or PCR analysis;
  • PCR product analysis and cloning control;
  • support for molecular genetics and genomics research.

Key technical specifications

  • 4-capillary capillary electrophoresis system;
  • applications: DNA sequencing, resequencing, and fragment analysis;
  • fluorescence-based detection of DNA fragments;
  • actual throughput depends on capillary configuration, polymer, sequencing chemistry, software, and run parameters.

Access and preparation

Before work begins, the analysis type, number of samples, quality and concentration of DNA or PCR product, primers, dyes or markers for fragment analysis, sample cleanup requirements, and result delivery format are agreed. For Sanger sequencing and fragment analysis, the quality of sample preparation is especially important.

Agilent 1260 Infinity II / LC MSD iQ HPLC-MS System for Qualitative and Quantitative Analysis of Liquid Samples

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Agilent 1260 Infinity II / LC MSD iQ HPLC-MS System for Qualitative and Quantitative Analysis of Liquid Samples

The high-performance liquid chromatography system with a mass spectrometric detector (HPLC-MS) combines chromatographic separation of complex mixtures with mass-based identification and quantitative analysis. It is a versatile platform for the study of bioactive compounds, plant extracts, pharmaceutical substances, surfactants, organic compounds, and selected classes of inorganic analytes in liquid samples.

Capabilities and typical tasks

  • qualitative and quantitative determination of compounds in complex liquid samples;
  • characterization and identification of substances based on mass spectrometric behavior;
  • LC-MS profiling of bioactive compounds and plant raw materials;
  • comparison and reproducibility assessment of chromatographic profiles of samples or extracts;
  • development and optimization of HPLC/HPLC-MS methods for routine and research analysis.

Key technical specifications

  • HPLC platform: Agilent 1260 Infinity II for analytical HPLC and entry-level UHPLC workflows;
  • quaternary pump: flow rate up to 10 mL/min and pressure up to 600 bar depending on operating range and configuration;
  • mass detector: LC MSD iQ, a single-quadrupole mass spectrometric detector integrated with liquid chromatography;
  • typical analytical use: liquid samples, extracts, pharmaceutical substances, surfactants, organic compounds, and selected inorganic analytes;
  • output: chromatograms, extracted ion chromatograms, mass spectra, qualitative identification, and quantitative reports depending on method setup.

Access and preparation

Before work begins, the research objective, sample composition and solvent, sample preparation requirements, sample compatibility with the chromatographic system, required columns, reagents, consumables, instrument operating mode, and result delivery format are agreed. For non-standard samples or new methods, preliminary technical feasibility assessment and method development or adaptation may be required.

Thermo Scientific Dionex UltiMate 3000 HPLC System for Analytical and Preparative Purification of Biomolecules

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Thermo Scientific Dionex UltiMate 3000 HPLC System for Analytical and Preparative Purification of Biomolecules

Dionex UltiMate 3000 is a liquid chromatography system for high-performance analytical and preparative separation of biomolecules. It enables purification, fractionation, purity control, and sample preparation for downstream research in molecular biology, biochemistry, and biotechnology.

Capabilities and typical tasks

  • analytical and preparative HPLC purification of biomolecules;
  • separation of components in complex biological mixtures;
  • control of chromatographic profiles and fraction purity;
  • optimization of elution conditions and flow rate;
  • real-time biomolecule detection using a multi-wavelength detector.

Key technical specifications

  • pump system for stable operation under high-pressure conditions;
  • precise flow-rate control for improved chromatographic resolution;
  • multi-wavelength real-time detection of biomolecules;
  • applications: analytical HPLC, semi-preparative purification, fractionation, and preparation of biomolecules for downstream research;
  • actual operating modes depend on the installed modules, columns, detector, and method configuration.

    • Access and preparation

    Before work begins, the sample type, buffer system, column, elution mode, expected fraction volume, detection requirements, fraction collection format, and downstream use of the obtained material are agreed. For new or complex samples, preliminary optimization of separation conditions may be required.

    Thermo Scientific Sorvall WX 80 Ultra Ultracentrifuge for Fractionation of Biomolecules and Cellular Components

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    Thermo Scientific Sorvall WX 80 Ultra Ultracentrifuge for Fractionation of Biomolecules and Cellular Components

    The ultracentrifuge is designed for high-speed sedimentation, density-gradient separation, purification, concentration, and fractionation of biological material. It is used for work with DNA, RNA, proteins, ribonucleoprotein complexes, subcellular fractions, and other biological macromolecules and particles.

    Capabilities and typical tasks

    • high-speed sedimentation of biological macromolecules and particles;
    • density-gradient separation of samples;
    • preparative isolation and purification of biomolecules;
    • preparation of subcellular fractions, protein complexes, and nucleic acids;
    • use of different rotor types according to the approved protocol.

    Key technical specifications

    • maximum platform specifications: up to 80,000 rpm and up to 602,640 × g;
    • temperature range: 0…+40 °C;
    • temperature control accuracy: ±0.5 °C;
    • speed range: from 1,000 rpm in 100 rpm increments;
    • speed control accuracy: ±10 rpm;
    • acceleration/deceleration profiles: 10/11;
    • microprocessor control;
    • actual operating modes are determined by the installed rotors; available rotors: fixed-angle T-647.5 — up to 47,500 rpm, 250,260 × g, 6 × 100 mL; swinging-bucket AH-629 — up to 29,000 rpm, 151,240 × g, 6 × 36 mL.

    Access and preparation

    Before work begins, the sample type, volume, buffer, required rotor, tube type, centrifugation mode, temperature, duration, balancing requirements, and safety requirements are agreed. The user must provide information about any potential biological, chemical, or other hazards associated with the samples. Work is performed only according to an approved protocol and using compatible consumables.

    Molecular Imager PharosFX Plus for Fluorescence and Phosphor-Screen Molecular Imaging

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    Molecular Imager PharosFX Plus for Fluorescence and Phosphor-Screen Molecular Imaging

    Molecular Imager PharosFX Plus is a molecular imaging system for acquisition and quantitative analysis of fluorescent and radioisotope-labeled signals from gels, membranes, phosphor screens, and other carriers. The instrument supports documentation and analysis of electrophoresis, blotting, and other molecular biology methods.

    Capabilities and typical tasks

    • single- and multicolor fluorescence imaging with direct laser excitation;
    • detection of DNA, RNA, and proteins in gels, blots, or microplates;
    • imaging of radioactively labeled samples using phosphor screens;
    • documentation of gels with colorimetric dyes;
    • quantitative signal analysis across a broad dynamic range.

    Key technical specifications

    • high-sensitivity and high-resolution fluorescence imaging support;
    • use of customized emission filters for different fluorophores;
    • quantification of 32P, 33P, 35S, 14C, and 3H across a broad dynamic range;
    • compatibility with 20 × 25 cm and 35 × 43 cm phosphor screens;
    • Quantity One® 1-D software with templates for common fluorophores and dyes.

    Access and preparation

    Before work begins, the carrier type, signal type, fluorophores or labels, required filters, scanning mode, exposure parameters, file format, and requirements for quantitative analysis are agreed. When working with radioisotope-labeled samples, radiation safety requirements and internal rules for handling such materials are additionally taken into account.

    BD FACSCalibur Flow Cytometer for Multiparametric Cell Analysis

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    BD FACSCalibur Flow Cytometer for Multiparametric Cell Analysis

    BD FACSCalibur is a laser-based flow cytometer for multiparametric analysis of cells and particles in suspension. The instrument enables assessment of light scatter, fluorescence labeling, immunophenotype, cell subpopulations, marker expression, and cell viability.

    Capabilities and typical tasks

    • FSC/SSC analysis for assessment of cell size and granularity;
    • fluorescence analysis of cells and particles depending on the actual instrument configuration;
    • immunophenotyping of cell populations;
    • assessment of marker expression, cell viability, and apoptosis;
    • analysis of cellular and particle suspensions in cell biology research.

    Key technical specifications

    • automated benchtop flow cytometry system;
    • the laser, detector, optical-filter, and available fluorescence-channel configuration is determined by the actual instrument setup and should be confirmed during study planning;
    • multiparametric analysis of cells and particles within the actual channel and filter configuration.

    Access and preparation

    Before analysis, the type of cells or particles, buffer, suspension concentration, fluorescent labels, antibody panel, control samples, event acquisition parameters, and data analysis format are agreed. Samples must be prepared according to method and safety requirements, and work with potentially hazardous material requires separate approval.

    Bio-Rad ChemiDoc XRS+ System for Gel Documentation, Chemiluminescence and Fluorescence Imaging

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    Bio-Rad ChemiDoc XRS+ System for Gel Documentation, Chemiluminescence and Fluorescence Imaging

    Bio-Rad ChemiDoc XRS+ is a digital imaging system for documentation and quantitative analysis of gels, membranes, and blots. The instrument is used for chemiluminescent, fluorescent, colorimetric, and UV/visible signal detection in molecular biology and biochemical studies.

    Capabilities and typical tasks

    • chemiluminescent imaging of western blots;
    • documentation of DNA/RNA gels and protein gels;
    • fluorescent and colorimetric detection depending on configuration;
    • quantitative signal analysis using Image Lab software;
    • high-sensitivity imaging for comparative analysis.

    Key technical specifications

    • supercooled, supersensitive 16-bit CCD camera;
    • support for chemiluminescent, fluorescent, and colorimetric detection;
    • applications: western blot, DNA/RNA gels, protein gels, membranes, and other carriers;
    • automated exposure setup and image processing in Image Lab depending on the method;
    • actual light sources and filters should be verified according to the configuration.

    Access and preparation

    Before work begins, the carrier type, detection method, dye or substrate, imaging mode, exposure parameters, need for quantitative analysis, and file format are agreed. To obtain reproducible results, gel, membrane, or blot preparation conditions and requirements for subsequent image processing should be agreed in advance.